Composite

Part:BBa_K3453170:Design

Designed by: Maeva Cherriere   Group: iGEM20_Evry_Paris-Saclay   (2020-10-24)


Grapevine VvTrnL-UAA Toehold Switch 1.1 with sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 53
    Illegal SapI.rc site found at 138


Design Notes

T7 promoter + Grapevine VvTrnL-UAA Toehold Switch 1.1 (BBa_K2916050) + sfGFP coding sequence (BBa_I746916) + scar + the BBa_B0015 Terminator


Source

DNA synthesis & Assembly

References